EchoLUTION Plant DNA 96 Kit (2 x 96)
- Order number: 010-103-002
- Product weight: 0.825 kg
For single-step 96-well plate-based purification of genomic DNA from up to 50 mg plant tissue tissue (leaves, soft roots, seeds, fruit tissue, bud & blossom tissue)
- High DNA yield: up to 5-fold more than silica plant kits
- High purity: reliable removal of inhibitors for downstream reactions (e. g., PCR, NGS)
- Convenience: no tedious bind-wash-elute procedure
- Speed: Whole process complete in 60 min
- Sustainability: > 50 % less plastic than with Silica-based methods, no hazardous liquids
Kit contents
Microtube 8-Strips & Lids for lysis, Purification Plates, DNA Elution Plates, Adhesive Foils, Beating Buffer P (BB), Plant Lysis Buffer, TurboLyse Protease Mix (P), RNase A (R), Clearing Solution (CS), Tris Buffer,
EchoLUTION Plant DNA Kit (2 x 96) Product no.: Product no. 010-103-002
For single-step 96-well plate-based purification of genomic DNA from up to 50 mg of plant tissue (leaves, soft roots, seeds, fruit tissue, bud & blossom tissue)
The EchoLUTION Plant DNA Kit (96) has been developed to quickly purify genomic DNA from various species and types of tissues, including leaf, seed, fruit and root material (see table) with high throughput. No optimization of parameters like lysis time, temperature or elution volume is necessary.
The innovative EchoLUTION process in 96-well format does not need any washing steps and works completely in the absence of denaturing salts and organic solvents. These reagents - used in common Silica-based bind-wash-elute DNA extraction - inhibit enzymatic-reaction based analysis like PCR or NGS when carried over into the DNA preparation. The increase of overall speed, due to time savings from the process itself as well as the less error-prone enzymatic downstream analysis, makes EchoLUTION Plant DNA extraction kits the ideal tool for use in plant breeding projects.
Procedure
Duration: Done in 60 minutes, including bead beating, tissue lysis and single-step column purification.
Typically the 96-well procedure starts with homogenization of the plant samples by bead beating in the lysis plate (Beating buffer provided, please order steel beads separately). The subsequent lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. BioEcho’s TurboLyse P Protease Mix (patent pending) contains a unique combination of several active enzymes with an expanded substrate specificity. All kinds of plant tissues can be lysed fast and successfully – simply and with the same protocol.
The EchoLUTION 96-well plate-based purification procedure combines an initial filtration, holding back potential cell debris, with an extremely efficient reverse purification within just one step. Following the lysis step, after addition of Clearing solution, the lysate is centrifuged, and the supernatant loaded onto the previously conditioned Purification Plate. The entire process is done after one additional centrifugation step. The purified high molecular weight genomic and mitochondrial DNA is eluted, while all debris and cellular compartments, inhibitors, small fragments (< 50 bp), and salts are completely removed. Since impurities are held back by the purification matrix and since the highly pure DNA is collected in the flow-through, the complete DNA is easily recovered with close to 100 % recovery and highest purity. Addition of carrier nucleic acids is not required since matrix interactions have been minimized.
Applications
For a molecular breeding project, various vegetables and fruits need to be purified in sufficient amounts with reliable high purity for later detection in qPCR or NGS. The EchoLUTION Plant DNA 96 workflow can be easily performed in high throughput.
The data set below, kindly provided by a Molecular Breeding Company (The Netherlands), show 8 different leaf tissues (4 samples each) from the indicated origin. Both OD-based purity indicators are in the optimal range. DNA is of consistent high purity and was reliably detected in qPCR.
Plant Breeding Project. 4 DNA preparations each purified with EchoLUTION Plant DNA 96 Kit from 8 different vegetable or fruit leaf punches were spectrophotometrically analyzed.
All samples were detected with high yield and optimal purity ratios (A260/280 and A260/230).
EchoLUTION-purified DNA comprises of particularly long DNA fragments, free of contaminants and enzyme inhibitors like chaotropic reagents and organic solvents and is highly suitable for all downstream applications such as PCR, Genotyping and NGS.
EchoLUTION Plant DNA Kit (2 x 96) Product no.: Product no. 010-103-002
For single-step 96-well plate-based purification of genomic DNA from up to 50 mg of plant tissue (leaves, soft roots, seeds, fruit tissue, bud & blossom tissue)
The EchoLUTION Plant DNA Kit (96) has been developed to quickly purify genomic DNA from various species and types of tissues, including leaf, seed, fruit and root material (see table) with high throughput. No optimization of parameters like lysis time, temperature or elution volume is necessary.
The innovative EchoLUTION process in 96-well format does not need any washing steps and works completely in the absence of denaturing salts and organic solvents. These reagents - used in common Silica-based bind-wash-elute DNA extraction - inhibit enzymatic-reaction based analysis like PCR or NGS when carried over into the DNA preparation. The increase of overall speed, due to time savings from the process itself as well as the less error-prone enzymatic downstream analysis, makes EchoLUTION Plant DNA extraction kits the ideal tool for use in plant breeding projects.
Procedure
Duration: Done in 60 minutes, including bead beating, tissue lysis and single-step column purification.
Typically the 96-well procedure starts with homogenization of the plant samples by bead beating in the lysis plate (Beating buffer provided, please order steel beads separately). The subsequent lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. BioEcho’s TurboLyse P Protease Mix (patent pending) contains a unique combination of several active enzymes with an expanded substrate specificity. All kinds of plant tissues can be lysed fast and successfully – simply and with the same protocol.
The EchoLUTION 96-well plate-based purification procedure combines an initial filtration, holding back potential cell debris, with an extremely efficient reverse purification within just one step. Following the lysis step, after addition of Clearing solution, the lysate is centrifuged, and the supernatant loaded onto the previously conditioned Purification Plate. The entire process is done after one additional centrifugation step. The purified high molecular weight genomic and mitochondrial DNA is eluted, while all debris and cellular compartments, inhibitors, small fragments (< 50 bp), and salts are completely removed. Since impurities are held back by the purification matrix and since the highly pure DNA is collected in the flow-through, the complete DNA is easily recovered with close to 100 % recovery and highest purity. Addition of carrier nucleic acids is not required since matrix interactions have been minimized.
Applications
For a molecular breeding project, various vegetables and fruits need to be purified in sufficient amounts with reliable high purity for later detection in qPCR or NGS. The EchoLUTION Plant DNA 96 workflow can be easily performed in high throughput.
The data set below, kindly provided by a Molecular Breeding Company (The Netherlands), show 8 different leaf tissues (4 samples each) from the indicated origin. Both OD-based purity indicators are in the optimal range. DNA is of consistent high purity and was reliably detected in qPCR.
Plant Breeding Project. 4 DNA preparations each purified with EchoLUTION Plant DNA 96 Kit from 8 different vegetable or fruit leaf punches were spectrophotometrically analyzed.
All samples were detected with high yield and optimal purity ratios (A260/280 and A260/230).
EchoLUTION-purified DNA comprises of particularly long DNA fragments, free of contaminants and enzyme inhibitors like chaotropic reagents and organic solvents and is highly suitable for all downstream applications such as PCR, Genotyping and NGS.
- Get more information about the new EchoLUTION 96-well format
- Product Profile
- Advertorial in EBT - EchoLUTION 96-well formats
- Protocol
SDS (MSDS) saftey data sheet (material safety data sheet), please request: contact@bioecho.de
Dr. Yang Yi , Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences
''We compared the EchoLUTION Plant DNA Kit with our actual silica-technology based kit. Using BioEcho´s Kit we obtained the genomic DNA in high yields much faster and easier than before. We were impressed about the simplicity of the whole process.''
Jérôme Morinière (CEO) & Dr. René Tänzler (CTO), Advanced Identification Methods – AIM GmbH, Munich, Germany
"We at Advanced Identification Methods (AIM) are specialized on the analyses of the biodiversity of our environment using genomic methods. E. g., we analyze bulk samples deriving from insect traps, mixed plant, wood and soil samples by extracting the total DNA from the biological samples and a subsequent identification of individuals present by DNA metabarcoding. We have thoroughly tested BioEcho’s EchoLUTION DNA extractions kits for animal tissue and plant materials and use these very successfully in our routine workflows. Besides the technical reliability of the kits, we like BioEcho’s sustainability approach a lot. Using their kits, we save a lot of plastic waste and don’t have any hazardous liquid waste any more!"
Eva Gertman, , Carlsberg Research Laboratory, Copenhagen, Denmark
"We have tested and have very good experiences with BioEcho’s Plant 96 kit. The consumption of plastic is less and the extraction process is faster and simpler than before on our Cybio-Felix robot. We have had a really good collaboration with BioEcho and their local distributor Kem-En-Tec Nordic, who have been very flexible and helpful with the setup and automation process.”
Dr. Yang Yi , Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences
''We compared the EchoLUTION Plant DNA Kit with our actual silica-technology based kit. Using BioEcho´s Kit we obtained the genomic DNA in high yields much faster and easier than before. We were impressed about the simplicity of the whole process.''
Jérôme Morinière (CEO) & Dr. René Tänzler (CTO), Advanced Identification Methods – AIM GmbH, Munich, Germany
"We at Advanced Identification Methods (AIM) are specialized on the analyses of the biodiversity of our environment using genomic methods. E. g., we analyze bulk samples deriving from insect traps, mixed plant, wood and soil samples by extracting the total DNA from the biological samples and a subsequent identification of individuals present by DNA metabarcoding. We have thoroughly tested BioEcho’s EchoLUTION DNA extractions kits for animal tissue and plant materials and use these very successfully in our routine workflows. Besides the technical reliability of the kits, we like BioEcho’s sustainability approach a lot. Using their kits, we save a lot of plastic waste and don’t have any hazardous liquid waste any more!"
Eva Gertman, , Carlsberg Research Laboratory, Copenhagen, Denmark
"We have tested and have very good experiences with BioEcho’s Plant 96 kit. The consumption of plastic is less and the extraction process is faster and simpler than before on our Cybio-Felix robot. We have had a really good collaboration with BioEcho and their local distributor Kem-En-Tec Nordic, who have been very flexible and helpful with the setup and automation process.”