EchoLUTION Blood DNA Micro Kit (250)

EchoLUTION Blood DNA Micro Kit (250)   Product no.: 010-001-250

For single-step spin column purification from up to 60 µl liquid blood or dried blood spots (FTA cards)

The EchoLUTION Blood DNA Kit has been developed to purify genomic and mitochondrial DNA from all kind of blood including fresh, frozen or whole blood or dried blood spots (FTA card punches). EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used.

Procedure:

Duration:  approx. 30 minutes, including blood lysis and single-step column purification (see flow-chart).

The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. BioEcho’s TurboLyse B&C Protease Mix contains a unique combination of several active enzymes with an expanded substrate specificity. Blood cells (from, e. g., human, cat and cow origin), also dried blood spots (coated FTA cards) are lysed fast and successfully – simple and with the same protocol.

The subsequent EchoLUTION single-step spin purification procedure combines an initial filtration, holding back potential cell debris, with an extremely efficient reverse purification. The cleared lysate is directly loaded onto the spin column. After just one single spin, the purified high molecular weight genomic and mitochondrial DNA is eluted, while all debris and cellular compartments, inhibitors, small fragments (< 300 bp), and salts are completely removed. Since impurities are held back by the purification matrix and since the highly pure DNA is collected in the flow-through, the complete DNA is easily recovered with close to 100 % recovery and highest purity (see fig).

This convenient procedure requires none of the tedious bind-wash-elute steps of common Silica-based procedures and enables a significantly faster purification of nucleic acid samples – with less handling, drastically reduced plastic consumable usage and no use of hazardous materials like chaotropic salts or organic solvents (see figure).

Plastic waste produced from a 50 reactions Silica-based DNA kit and from an EchoLUTION kit (including kit components and consumables that are not part of the kit). Bags contained in BioEcho kits are cellophane-based. In numbers:
 

Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for downstream applications (see below). The amount obtained is up to 2-fold increased per µl blood compared to common silica methods. Protector Solution is supplied to enable long-term storage of DNA samples. EchoLUTION purified genomic DNA preparations usually show an A260/280 ratio of around 1.8.

Applications

Since EchoLUTION kit purified DNA do not contain any traces of chaotropic salts, organic solvents or EDTA, a superior performance is seen in downstream applications such as real-time PCR or PCR, even with undiluted samples with the DNA solution making up to 45% of the total PCR reaction volume. BioEcho-purified DNA comprises of particularly long DNA fragments, free of contaminants and enzyme inhibitors like chaotropic reagents and organic solvents and is highly suitable for all downstream applications such as PCR, Genotyping and NGS applications.