For the one step isolation of genomic DNA from 1 ml of gram- and gram+ bacterial culture.
Duration: approx. 25 minutes (gram - bacteria), or 45 minutes (Gram + bacteria) including lysis and one step column purification.
Yields are depending on bacteria species and culturing conditions. The isolated genomic DNA will also contain plasmid DNA, if present in the bacteria.
EchoLUTION kits are based on the principle of negative chromatography, where impurities like salt, detergent und proteins are removed by the column material and the purified genomic DNA is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage and no use of hazardous materials like chaotropic salts or organic solvents.
The recovery of nucleic acids when using EchoLUTION kits is close to 100% since there is no loss of DNA or RNA caused by the binding to a carrier material. In comparison, binding to e.g. a silica membrane in bind-wash elute procedures will cause a loss of up to 30% of the target nucleic acid material. Also, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. Since EchoLUTION kits do not contain any chaotropic salts, organic solvents or EDTA, a superior performance is seen in real-time PCR, even with undiluted samples.
The purified genomic DNA is eluted in water and can immediately be used for downstream applications. A 20x TE Buffer is supplied to enable storage of samples in TE Buffer, pH 8.5.
EchoLUTION purified genomic DNA samples usually show an A260/280 ratio of around 1.8.
Spin columns, buffers, reagents, protease and RNAse A for one-step purification of genomic DNA from bacteria.